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中国癌症防治杂志

中国癌症防治杂志 ›› 2012, Vol. 4 ›› Issue (2): 170-173.doi: 10.3969/j.issn.1674-5671.2012.02.18

• 基础研究 • 上一篇    下一篇

舌癌耐药细胞株的建立及癌干细胞特性的实验观察

  

  1. 广西医科大学病理生理学教研室；广西医科大学研究生学院；广西医科大学实验中心
  • 出版日期:2012-06-25 发布日期:2012-07-03
  • 通讯作者: 邝晓聪 E-mail:13617715826@139.com
  • 基金资助:

    国家自然科学基金资助项目（30860326）；广西自然科学基金资助项目（0991139）；广西科技创新能力与条件建设项目（桂科攻0993003C-1）

Establishment of a drug-resistant tongue cancer cell line and analysis of its cancer stem cell characteristics

  • Online:2012-06-25 Published:2012-07-03

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被引次数

5

摘要： 目的 建立舌癌耐药细胞株，并观察耐药细胞株癌干细胞的相关生物学特性。方法 以低浓度阿霉素持续增量诱导法建立TCA-8113舌癌耐药细胞株，并对亲代与舌癌耐药细胞株进行癌细胞基本特性、癌干细胞相关细胞学特性的检测，分别以计数法检测癌细胞生长曲线、倍增时间以及用MTT法测定耐药倍数，并进行单个细胞成克隆实验、平板克隆形成实验和悬浮球形成实验。结果 TCA-8113舌癌耐药细胞为“铺路石”状上皮样生长方式，但其中异形细胞略多，耐药倍数为22倍；生长曲线提示其增殖速度明显低于亲本TCA-8113舌癌细胞，且倍增时间增加，但在细胞周期中各期细胞比例未见明显改变；TCA-8113舌癌耐药细胞克隆形成率、单个细胞培养成株率均明显低于其亲本TCA-8113舌癌细胞（P﹤0.05）。结论 低浓度阿霉素持续增量法可诱导形成舌癌耐药细胞株，但舌癌耐药细胞株的癌干细胞特征并不明显。

关键词:  , 舌癌, 耐药细胞, 癌干细胞, 耐药诱导, 特性

Abstract: Objective To establish a drug-resistant tongue cancer cell line and analyze its cancer stem cell（CSC）characteristics. Methods Tongue cancer cell line TCA-8113 was induced by adriamycin（ADM）and used to establish the drug-resistant cell line TCA-8113/ADM.Basic characteristics of both the parental cell line TCA-8113 and the drug-resistant cell line TCA-8113/ADM were analyzed and compared， including cell growth，doubling time，cell cycle， resistance index（RI），single-cell culture，colony formation and formation of sphere cells. Results The growth pattern of TCA-8113/ADM cells was cobblestone-like， with cultures showing sig-nificantly more atypical cells than those of parental cells.TCA-8113/ADM cells had an RI of 22，and their rates of cell proliferation， cell doubling，colony formation and sphere cell formation were slower than those of the parental cells. Conclusion Sustained incre-mental exposure to low concentrations of ADM can induce a drug-resistant tongue cancer cell line，but its CSC characteristics are not obvious.

Key words: Tongue cancer, Drug-resistant, Cancer stem cell, Drug-resistant inducement, Characteristic